A Feasibility Study on the Use of a Structured Light Depth-Camera for Three-Dimensional Body Measurements of Dairy Cows in Free-Stall Barns

Frequent checks on livestock\u2019s body growth can help reducing problems related to cow infertility or other welfare implications, and recognizing health\u2019s anomalies. In the last ten years, optical methods have been proposed to extract information on various parameters while avoiding direct contact with animals\u2019 body, generally causes stress. This research aims to evaluate a new monitoring system, which is suitable to frequently check calves and cow\u2019s growth through a three-dimensional analysis of their bodies\u2019 portions. The innovative system is based on multiple acquisitions from a low cost Structured Light Depth-Camera (Microsoft Kinect\u2122 v1). The metrological performance of the instrument is proved through an uncertainty analysis and a proper calibration procedure. The paper reports application of the depth camera for extraction of different body parameters. Expanded uncertainty ranging between 3 and 15 mm is reported in the case of ten repeated measurements. Coef\ufb01cients of determination R2> 0.84 and deviations lower than 6% from manual measurements where in general detected in the case of head size, hips distance, withers to tail length, chest girth, hips, and withers height. Conversely, lower performances where recognized in the case of animal depth (R2 = 0.74) and back slope (R2 = 0.12)

Editorial: Precision livestock farming: a 'per animal' approach using advanced monitoring technologies

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Acoustic analysis of some characteristics of red deer roaring

Different strategies have been adopted to track and monitor red deer (<em>Cervus elaphus</em>) populations according to different habitats and ecosystems. The population census techniques usually carried out are time consuming and involve lot of manpower. A method that relies on individual animal signs like acoustic indices could be useful to support the wildlife management, since vocalisations can encode and transmit a variety of biologically significant information. The present study considered 460 roars recorded from 14 adult deers, from five to eight years old. Recordings were performed in five different locations of northern-central Italy. The acoustic spectrum of each roar was analysed in order to extract its main features, namely: the fundamental frequency (F0), the peak frequency (PF), the sound length (SL) and 24 frequency bins of 174.3 Hz, representing the sound distribution between 50 and 4233.2 Hz (F1 to F24). Statistical analyses showed that individuality and age of animals were significant on F0 (P0.90; P&lt;0.001), indicating that age could influence the spectral features of roaring. The roaring frequency variables appear to be indicators of the individuality of male deer, even if the strong influence of age on the emitted sounds could compromise the reliability of the method over long periods of time

The use of image analysis as a new approach to assess behaviour classification in a pig barn

The aim of this study was to develop an innovative method for measuring the activity level of pigs in a barn in real time. An infrared-sensitive camera was placed over two pens of the piggery, images were recorded for 24 h a day for eight days during the fattening period, and the activity and occupation indices were calculated every second in real time using software. In the laboratory, the recorded images were visually labelled to score the animals' behaviour adopting the Martin and Bateson's Scan sampling method, and to find a relation with the automatically measured activity index. Pigs' behaviour was assigned in the following way, 0 - no activity, 1 - fighting or struggling, 2 - biting one another, 3 - abnormal behaviour as nuzzling or suckling one another (interacting pigs), 4 - feed assumption time. Pigs spent most of the time lying inactively (82–90% of the time), following a diurnal rhythm with peaks related to the feeding administration routine; fighting episodes occurred very rarely (0.05–0.10% of the time). Based on the analysis of the automatically measured group activity index compared to the manual labelling, a relation was found between the activity index and the behaviour types (no activity, nuzzling and feeding). The novelty presented in this study was the development of on-line tools to monitor farm animals continuously during their life, in a fully automatic way, with objective measures and criteria without imposing additional stress to the animals

Effects of disinfectant fogging procedure on dust, ammonia concentration, aerobic bacteria and fungal spores in a farrowing-weaning room

Introduction and Objective. In the last decades, large-scale swine production has led to intensive rearing systems in which air quality can be easily degraded by aerial contaminants that can pose a health risk to the pigs and farm workers. This study evaluated the effects of fogging disinfectant procedure on productive performance, ammonia and dust concentration, aerobic bacteria and fungal spores spreading in the farrowing–weaning room. Materials and Method. This trial was conducted in 2 identical farrowing-weaning rooms of a piggery. In both rooms, 30 pregnant sows were lodged in individual cages. At 75 days of age, the piglets were moved to the fattening room. In the treated room, with the birth of the first suckling-pig, the fogging disinfection with diluted Virkon S was applied once a day in the experimental room per 15 minutes at 11:00. The fogging disinfectant treatment was switched between rooms at the end of the first trial period. Temperature, relative humidity, dust (TSP-RF fractions and number of particles), ammonia concentration and aerial contaminants (enterococci, Micrococcaeae and fungal spores) were monitored in both rooms. Results. Ammonia concentration reduction induced by fogging disinfection was estimated 18%, total suspended particles and the respirable fraction were significantly lower in the experimental room. Fungal spores resulted in a significant reduction by the fogging procedure, together with dust respirable fraction and fine particulate matter abatement. Conclusions. The fogging disinfection procedure improved air quality in the piggery, thereby enhancing workers and animals health

The influence on biogas production of three slurry-handling systems in dairy farms

Handling systems can influence the production of biogas and methane from dairy farm manures. A comparative work performed in three different Italian dairy farms showed how the most common techniques (scraper, slatted floor, flushing) can change the characteristics of collected manure. Scraper appears to be the most neutral choice, as it does not significantly affect the original characteristics of manure. Slatted floor produces a manure that has a lower methane potential in comparison with scraper, due to: a lower content of volatile solids caused by the biodegradation occurring in the deep pit, and a lower specific biogas production caused by the change in the characteristics of organic matter. Flushing can produce three different fluxes: diluted flushed manure, solid separated manure and liquid separated manure. The diluted fraction appears to be unsuitable for conventional anaerobic digestion in completely stirred reactors (CSTR), since its content of organic matter is too low to be worthwhile. The liquid separated fraction could represent an interesting material, as it appears to accumulate the most biodegradable organic fraction, but not as primary substrate in CSTR as the organic matter concentration is too low. Finally, the solid-liquid separation process tends to accumulate inert matter in the solid separated fraction and, therefore, its specific methane production is low

livestock precision farming and the kit radicaux libres test two simple approaches to detect variation in piglets behaviour and welfare

In commercial practice, the mixing of pigs from different litters is very frequently done in order to minimise the within-pen variation in weight. Aggressive behavior can be easily observed when unknown pigs are mixed into a group. Post-mixing fighting occurs between pigs that are unfamiliar with each other and some times leads to serious injury. For the establishment of social hierarchy, aggression may occur. The aim of the study was to evaluate the response of piglets fed and not with dietary plant extract (PE) when exposed to mixing-induced stress. Behavioural and physiological indicators combined with Precision Livestock Farming (PLF) methods were used. A total of 84 female piglets (Dalland), (average live weight 5.53 ± 0.1 kg, corresponding to day 0 of experimental trial) divided into 6 pens of 14 piglets each were randomly assigned to two dietary treatments: a control diet (CON) and a diet supplemented with 5 mg/kg feed of plant extract (PE). At 37 d of the experimental trial, half the animals of two treated pens (7+7) and half the animals of two control pens (7+7) were mixed. The experimental trial lasted 59 days. Blood from piglets of each treatment was collected by anterior vena cava puncture before mixing, on day 37, and after mixing, on day 39, for assessing total blood antioxidant activity, by Kit Radicaux Libres (KRL) test and haptoglobin (HPT) and cortisol concentration as biological indicators of stress. Behaviour measurements were performed using an ethological rating scale and Precision Livestock Farming methods as indicator of qualitative behavioural assessment. Dietary treatment and mixing affected total antioxidant activity (P &lt; 0.05) of whole blood that was higher in the PE group and after mixing. A trend effect of mixing was found for cortisol and haptoglobin which were significantly (P &lt; 0.05) lower in the PE group. The rating scale and PLF showed significant variation in the abnormal animal behaviours due to mixing; continuous monitoring of pigs by PLF showed more (P &lt; 0.05) animal activity in the PE group than in CON. In this context, the behaviour measurements were demonstrated to be simple and reliable tools for evaluating pig behaviour and KRL test was an effective method for assessing antioxidant activity. </p

Automatic cough detection for bovine respiratory disease in a calf house

peer-reviewedIn calf rearing, bovine respiratory disease (BRD) is a major animal health challenge. Farmers incur severe economic losses due to BRD. Additional to economic costs, outbreaks of BRD impair the welfare of the animal and extra expertise and labour are needed to treat and care for the infected animals. Coughing is recognised as a clinical manifestation of BRD. Therefore, the monitoring of coughing in a calf house has the potential to detect cases of respiratory infection before they become too severe, and thus to limit the impact of BRD on both the farmer and the animal. The objective of this study was to develop an algorithm for detection of coughing sounds in a calf house. Sounds were recorded in four adjacent compartments of one calf house over two time periods (82 and 96 days). There were approximately 21 and 14 calves in each compartment over the two time-periods, respectively. The algorithm was developed using 445 min of sound data. These data contained 664 different cough references, which were labelled by a human expert. It was found that, during the first time period in all 3 of the compartments and during the second period in 2 out of 4 compartments, the algorithm worked very well (precision higher than 80%), while in the 2 other cases the algorithm worked well but the precision was less (66.6% and 53.8%). A relation between the number of calves diagnosed with BRD and the detected coughs is shown

Out of the deep: Cryptic speciation in a Neotropical gecko (Squamata, Phyllodactylidae) revealed by species delimitation methods

Levels of biodiversity in the Neotropics are largely underestimated despite centuries of research interest in this region. This is particularly true for the Cerrado, the largest Neotropical savanna and a formally recognized biodiversity hotspot. Molecular species delimitation methods have become essential tools to uncover cryptic species and can be notably robust when coupled with morphological information. We present the first evaluation of the monophyly and cryptic speciation of a widespread Cerrado endemic lizard, Gymnodactylus amarali, using phylogenetic and species-trees methods, as well as a coalescent-based Bayesian species delimitation method. We tested whether lineages resulting from the analyses of molecular data are morphologically diagnosed by traditional meristic scale characters. We recovered eight deeply divergent molecular clades within G. amarali, and two additional ones from seasonally dry tropical forest enclaves between the Cerrado and the Caatinga biomes. Analysis of morphological data statistically corroborated the molecular delimitation for all groups, in a pioneering example of the use of support vector machines to investigate morphological differences in animals. The eight G. amarali clades appear monophyletic and endemic to the Cerrado. They display several different properties used by biologists to delineate species and are therefore considered here as candidates for formal taxonomic description. We also present a preliminary account of the biogeographic history of these lineages in the Cerrado, evidence for speciation of sister lineages in the Cerrado–Caatinga contact, and highlight the need for further morphological and genetic studies to assess cryptic diversity in this biodiversity hotspot.FMCBD was supported by scholarships from ‘Coordenação de Aperfeiçoamento de Pessoal de Nível Superior’ (CAPES) and ‘Conselho Nacional de Desenvolvimento Científico e Tecnológico’ (CNPq). This work was funded by CAPES, CNPq, ‘Fundação de Apoio à Pesquisa do Distrito Federal’ (FAPDF), ‘GENPAC15’ and ‘Rede de Pesquisa Biota do Cerrado’, coordinated by GRC. MTR and JC acknowledge ‘Fundação de Amparo à Pesquisa do Estado de São Paulo’ (FAPESP) for funding and D. Pavan, F. Curcio, M. Dixo, M. Teixeira Jr., N. Silva P. Nunes and, T. Mott for collecting tissues and help in field. LBB thanks additional support provided by the Australian Research Council (ARC DP0556496). We are grateful to Fernanda Werneck for collecting the Gymnodactylus specimens of Manga and Matias Cardoso. We also acknowledge two anonymous reviewers for comments that improved the quality of the manuscript. FMCBD is also grateful to Marina Scalon for commenting on early drafts of the manuscript